[Study on UIPLC-DAD fingerprint of Disporum cantoniense]

To establish an UHPLC-DAD fingerprint of 30 batches of Miao medicine Disporum cantoniense from Guizhou, and to provide a theoretical evidence to evaluate its quality.

The analysis was carried out on an Agilent Eclipse ZOR-BAX Plus C18 (100 mm x 2. 1 mm, 1. 8 µm) column with a mobile phase consisting of acetonitrile-water(containing 0. 1% formic acid) with gradient elution, and the flow rate was 0. 3 mL/min. The column temperature was set at 40 °C and UV detection wavelength was set at 290 nm. The sample injection volume was 3 µL. The similarity evaluation and principal component analysis(PCA) of these fingerprints were carried out.

The UHPLC-DAD fingerprint was established and compared by 30 batches of samples similarity with 12 common peaks and 3 peaks were identified. The similarities of 30 batches of Disporum cantoniense were between 0. 766 and 0. 994. The principal component analysis showed that compounds 1 and 12 were representative for the fingerprint.

The method is accurate and credible,which can be used for identification and quality control of Disporum cantoniense.