Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Si-modified beta-Ti-25Mo biomaterials with reduced elastic modulus, enhanced corrosion resistance, and cytocompatibility.

Biomedical physics & engineering express·2026
Same author

Recent advances in the recognition of the explosive Mother of Satan: a review.

Analytical methods : advancing methods and applications·2026
Same author

<i>S. aromaticum</i> aqueous extract-derived carbon dots: a biosafe antibacterial nanocatalyst for water remediation.

Journal of materials chemistry. B·2026
Same author

3D Printed Angiogenin-Functionalized Bioresorbable Tubular Conduits for Biological Vascularization.

ACS applied bio materials·2026
Same author

Thiolated Chitosan-Stearic Acid Thiol-Linked Biomimetic Hydrogels for Axonal Regrowth and Neuronal Tissue Regeneration.

ACS applied bio materials·2026
Same author

3D-printed skeletal tissue analogues using bone decellularized extracellular Matrix with PCL-bioglass composite: A biomimetic approach.

Colloids and surfaces. B, Biointerfaces·2026

Related Experiment Video

Updated: Apr 11, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

1.3K

Orange-red silver emitters for sensing application and bio-imaging.

Mainak Ganguly1, Jayasmita Jana, Bodhisatwa Das

  • 1Department of Chemistry, Furman University, Greenville, South Carolina-29613, USA.

Dalton Transactions (Cambridge, England : 2003)
|June 2, 2015
PubMed
Summary

Highly fluorescent gold-silver (Au(I)@Ag) nanoparticles were synthesized for sensitive sulfide detection. These stable, cytocompatible nanoparticles enable live cell imaging and environmental sample analysis.

More Related Videos

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.8K
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

8.6K

Related Experiment Videos

Last Updated: Apr 11, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

1.3K
Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.8K
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

8.6K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Gold-silver (Au(I)@Ag) nanoparticles exhibit unique optical properties.
  • Silver clusters are known for their fluorescence, but often lack stability.
  • Developing stable fluorescent nanomaterials is crucial for advanced applications.

Purpose of the Study:

  • To synthesize highly fluorescent Au(I)@Ag nanoparticles.
  • To investigate their potential for live cell imaging.
  • To develop a sensitive method for sulfide ion detection.

Main Methods:

  • Synthesis of Au(I)@Ag nanoparticles using silver nitrate, gold(III) chloride, and glutathione.
  • Modified hydrothermolysis (MHT) reaction to form silver clusters.
  • Characterization of fluorescence properties and stability.
  • Testing for live cell imaging and selective sulfide ion detection.

Main Results:

  • Au(I)@Ag nanoparticles with high fluorescence (emission max at 635 nm) were successfully synthesized.
  • The Au(I) moiety enhanced the stability of fluorescent silver clusters.
  • The nanoparticles demonstrated cytocompatibility and efficacy in live cell imaging.
  • Selective and sensitive quenching of fluorescence by sulfide ions was observed, enabling detection.
  • Lead(II) ions facilitated sulfide detection free from common interferences.
  • Visual detection of sulfide ions was possible due to color change.

Conclusions:

  • Au(I)@Ag nanoparticles are stable, fluorescent, and cytocompatible, suitable for live cell imaging.
  • The developed method allows for sensitive and selective detection of sulfide ions in various solvents.
  • This approach offers a promising tool for real-world environmental monitoring of sulfide.