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Related Experiment Video

Updated: Feb 6, 2026

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain
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Array-in-well serodiagnostic assay utilizing upconverting phosphor label technology.

Minna Ylihärsilä1, Siina Alaranta2, Susanne Lahdenperä2

  • 1Department of Virology, University of Turku, Kiinamyllynkatu 13, FI-20520 Turku, Finland; Department of Biotechnology, University of Turku, Tykistökatu 6A, FI-20520 Turku, Finland.

Journal of Virological Methods
|June 2, 2015
PubMed
Summary

This study developed a multiplex serological assay for detecting IgG antibodies against parvovirus B19 and human adenovirus simultaneously. The novel array-in-well assay demonstrated high accuracy, offering an efficient new tool for multiplex serology.

Keywords:
AdenovirusArray-in-wellMultiplexParvovirus B19SerodiagnosticUpconverting phosphor

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Area of Science:

  • Immunology
  • Virology
  • Biotechnology

Background:

  • Simultaneous detection of antibodies against multiple pathogens is crucial for serodiagnostics.
  • Existing methods for detecting parvovirus B19 and human adenovirus IgG antibodies can be time-consuming and require separate tests.

Purpose of the Study:

  • To develop and evaluate a multiplex serological array-in-well assay for the simultaneous detection of serum IgG antibodies against parvovirus B19 and human adenovirus.
  • To assess the diagnostic accuracy of this novel assay compared to established methods.

Main Methods:

  • Construction of a multiplex array-in-well assay on streptavidin-coated 96-well plates.
  • Spotting of biotinylated parvovirus B19 virus-like particles and adenovirus type 2 and 5 hexon antigens.
  • Detection of bound antibodies using anti-human IgG antibody-coated photon upconverting nanoparticles and an anti-Stokes photoluminescence imager.

Main Results:

  • The assay demonstrated high agreement with reference methods: 100% for parvovirus B19 IgG and 96% for adenovirus IgG.
  • The multiplex array-in-well assay successfully detected specific antiviral IgG antibodies in human sera.
  • Upconverting phosphor reporter technology enabled sensitive detection in the array format.

Conclusions:

  • The developed array-in-well assay is an efficient and promising tool for multiplex serology.
  • This technology enables simultaneous detection of antiviral antibodies, improving serodiagnostic capabilities.
  • The assay represents a significant advancement in the field of serological diagnostics for viral infections.