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Updated: Apr 11, 2026
Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
Ligamentization of Autogenous Hamstring Grafts After Anterior Cruciate Ligament Reconstruction: Midterm Versus
Shikui Dong1, Guoming Xie1, Yang Zhang1
1Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Background:
In previous studies, unimodal, small-diameter collagen fibrils have been commonly observed as the final collagen ultrastructure of the implanted grafts used in anterior cruciate ligament (ACL) reconstruction. However, the native ACL and hamstring tendon show bimodal collagen fibril distribution, consisting of both large- and small-diameter collagen fibrils.
Hypothesis:
Bimodal collagen fibril distribution of the graft is a common phenomenon after ACL reconstruction with hamstring tendon grafts and is time dependent.
Study Design:
Controlled laboratory study.
Methods:
A total of 52 patients who underwent double-bundle ACL reconstruction using autogenous hamstring tendons and who also underwent second-look arthroscopic surgery were enrolled. The patients were divided into 2 groups according to the time interval between the 2 operations: the midterm group (27 patients), with a 13- to 30-month time interval between operations, and the long-term group (25 patients) with a 31- to 62-month interval. During the second-look arthroscopic procedures, ACL graft biopsies were performed. Normal ACL tissues were harvested from 9 patients who underwent total knee replacement, and biopsy specimens of the to-be-grafted semitendinosus tendon tissues were also harvested from another 9 patients who underwent ACL reconstruction with hamstring tendons, which were designated as normal controls. Graft vascularity, cellularity, metaplasia, cellular metabolism, and collagen fibril distribution were analyzed.
Results:
Large-diameter (>100 nm) collagen fibrils were detected in 81.5% of the specimens in the midterm group and in 68.0% of the specimens in the long-term group. A typical bimodal distribution mode was observed in 62.6% of the specimens in the midterm group and in 52.0% of the specimens in the long-term group. There was no significant difference between groups with respect to the presence of large-diameter collagen fibrils, bimodal distribution, graft vascularity, cellularity, metaplasia, or cellular metabolic status.
Conclusion:
Graft ultrastructural maturation, characterized by large-diameter collagen fibrils and a bimodal collagen fibril distribution, is a common phenomenon and is not time dependent in the midterm to long term.
Clinical Relevance:
After hamstring tendon ACL reconstruction, the implanted grafts can transform into ACL-like tissue with a similar ultrastructure and metabolism, implying their usefulness as grafts.
