Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

28.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.8K
DNA Base Pairing02:27

DNA Base Pairing

36.0K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
36.0K
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

1.7K
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kaposi's Sarcoma-Associated Herpesvirus Utilizes and Manipulates RNA N<sup>6</sup>-Adenosine Methylation To Promote Lytic Replication.

Journal of virology·2017
Same author

NOTE FROM THE EDITOR.

RNA (New York, N.Y.)·2016
Same author

Erratum: Preparation of Nuclear Extracts from HeLa Cells.

Cold Spring Harbor protocols·2016
Same author

Cotranslational microRNA mediated messenger RNA destabilization.

eLife·2016
Same author

Preparing Cellular DNA from Nuclei or Whole Cells.

Cold Spring Harbor protocols·2015
Same author

Removal of rRNA from Deproteinized, Phenol-Extracted Total RNA by Hybrid Selection.

Cold Spring Harbor protocols·2015

Related Experiment Video

Updated: Apr 11, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

10.0K

Nucleotide analog interference mapping.

Timothy W Nilsen

    Cold Spring Harbor Protocols
    |June 3, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Modification interference assays identify crucial RNA chemical groups. Nucleotide analog interference mapping (NAIM) maps functional sites by incorporating modified nucleotides and analyzing cleavage patterns.

    More Related Videos

    Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
    10:05

    Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin

    Published on: January 20, 2016

    8.7K
    Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
    08:33

    Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

    Published on: January 19, 2015

    9.4K

    Related Experiment Videos

    Last Updated: Apr 11, 2026

    Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
    12:35

    Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

    Published on: November 14, 2017

    10.0K
    Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
    10:05

    Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin

    Published on: January 20, 2016

    8.7K
    Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
    08:33

    Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

    Published on: January 19, 2015

    9.4K

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • RNA Biology

    Background:

    • Modification interference assays are powerful tools for identifying functionally critical chemical groups within RNA.
    • These methods analyze RNA modifications in the phosphodiester backbone or nucleobases.

    Purpose of the Study:

    • To describe the principles and applications of Nucleotide Analog Interference Mapping (NAIM) for RNA functional site identification.
    • To outline the methodology for performing NAIM, including its prerequisite thiophosphate interference analysis.

    Main Methods:

    • Modification interference assays involve reacting end-labeled RNAs with modifications at various positions.
    • Functional RNA molecules are separated from non-functional ones after a reaction of interest.
    • NAIM incorporates α-thionucleotides with modified bases into RNA, followed by iodoethanol cleavage to map modification sites.

    Main Results:

    • NAIM is effective when thiophosphate substitution alone does not inhibit the reaction.
    • Thiophosphate interference analysis is a necessary precursor to NAIM.
    • Unaffected positions by thiophosphate substitution are suitable for NAIM analysis.

    Conclusions:

    • NAIM is a valuable technique for precisely mapping functional sites in RNA molecules.
    • The method complements thiophosphate interference analysis, expanding the scope of modification interference studies.
    • Understanding RNA chemical group importance is crucial for deciphering RNA function.