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Updated: Apr 11, 2026

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
Published on: February 19, 2016
Detection of Pesticides and Metabolites Using Surface-Enhanced Raman Spectroscopy (SERS): Acephate
Susan L Clauson1, James M Sylvia, Thomas A Arcury
1EIC Laboratories, Inc., 111 Downey Street, Norwood, MA 02062 USA.
Abstract:
A protocol created for acephate detection on particulates and vapors surrounding farmworkers as well as in urine samples is reported. Acephate is detected to the low parts-per-billion (ppb) range using surface-enhanced Raman spectroscopy (SERS). Optimal SERS sensor metal choice and post-production treatments to improve sensor stability in aqueous solutions containing acephate are presented. Acephate is detected in the vapor phase and can be differentiated from urine components and structurally similar pesticides, including the acephate metabolite-degradation product methamidophos. Protocol evaluation and preliminary field tests from North Carolina farms are discussed.
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