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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
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A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry
Amandeep Kaur1, Mohammad A Haghighatbin, Conor F Hogan
1School of Chemistry, The University of Sydney, New South Wales, 2006, Australia. elizabeth.new@sydney.edu.au.
Chemical Communications (Cambridge, England)
|June 4, 2015
Abstract:
Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities – confocal microscopy, fluorescence lifetime imaging and flow cytometry.
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