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A modified method for the production of 36ClO3- for use in plant nitrate transport studies
J Ruiz-Cristin1, A J Chodera, D P Briskin
1Department of Agronomy, University of Illinois, Urbana 61801.
Analytical Biochemistry
|October 1, 1989
Abstract:
A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.