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Updated: Apr 11, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single-cell genome and transcriptome processing prior to high-throughput sequencing.

Ana M Aransay¹, Laura Barcena, Aintzane Gonzalez-Lahera

¹Genome Analysis Platform, CIC bioGUNE & CIBERehd, Technological Park of Bizkaia - Building 801A, 48160, Derio, Spain, amaransay@cicbiogune.es.

Methods in Molecular Biology (Clifton, N.J.)

|June 5, 2015

Summary

This summary is machine-generated.

Single-cell genome and transcriptome sequencing are crucial for understanding stem cell genetic diversity. This chapter details methods for preparing sequencing libraries from individual stem cells for DNA and mRNA analysis.

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Area of Science:

Genomics
Stem Cell Biology
Molecular Biology

Background:

Stem cell research requires understanding genetic variations within diverse cell populations.
Characterizing individual cells is key to unlocking stem cell potential.

Purpose of the Study:

To provide detailed protocols for single-cell library preparation.
To enable whole genome DNA and mRNA sequencing from single stem cells.

Main Methods:

Single-cell isolation techniques.
DNA library preparation for whole genome sequencing.
RNA library preparation for transcriptome sequencing.

Main Results:

Established protocols for generating high-quality sequencing libraries from single cells.
Demonstrated feasibility of whole genome and mRNA sequencing from individual stem cells.

Conclusions:

Single-cell genomics and transcriptomics offer powerful tools for stem cell research.
The described methods facilitate detailed genetic analysis of stem cell populations.