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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

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Related Experiment Video

Updated: Apr 11, 2026

Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique

Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique

Published on: December 12, 2025

Development of an effective and efficient DNA isolation method for Cinnamomum species.

B S Bhau¹, G Gogoi¹, D Baruah¹

¹Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.

|June 5, 2015

Summary

This study presents a modified DNA extraction method for Cinnamomum species, effectively removing polysaccharides and polyphenols. The improved protocol yields high-quality DNA suitable for various molecular biology applications.

Keywords:

Activated charcoal CTAB Cinnamomum DNA DNA isolation PCR reaction Polyvinyl pyrrolidone

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Area of Science:

Plant Molecular Biology
Biotechnology
Genomics

Background:

Cinnamomum species contain polysaccharides and secondary metabolites that impede DNA extraction.
High-quality DNA is essential for molecular biology studies, including genetic analysis and species identification.
Existing DNA extraction methods often struggle with recalcitrant plant tissues.

Purpose of the Study:

To develop a modified DNA extraction protocol for Cinnamomum and other polyphenol-rich plants.
To ensure high yield and purity of DNA for downstream molecular applications.
To validate the protocol's effectiveness on both lyophilized and non-lyophilized plant samples.

Main Methods:

A modified CTAB (Cetyl Trimethyl Ammonium Bromide) DNA extraction protocol was developed.
Higher concentrations of salt and activated charcoal were incorporated to eliminate polysaccharides and polyphenols.
The protocol was tested on various woody species, including four Cinnamomum species.

Main Results:

The modified protocol successfully extracted high-quality and high-quantity DNA from diverse plant samples.
Extracted DNA demonstrated excellent amplification in Random Amplified Polymorphic DNA (RAPD) analysis.
DNA barcoding primers and restriction digestion confirmed the integrity and usability of the extracted DNA.

Conclusions:

The developed protocol is effective for DNA extraction from plants rich in polysaccharides and polyphenols.
This reproducible method is suitable for various plant molecular biology studies, including genetic research and species identification.
The protocol offers a reliable solution for obtaining high-quality DNA from challenging plant materials.