Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

3.7K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
3.7K
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

743
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
743

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging Challenges: High frequency of Antiseptic Resistance Encoding Genes and Reduced Biguanide Susceptibility in Antibiotic-Resistant <i>Acinetobacter baumannii</i> in Iran.

Archives of Razi Institute·2025
Same author

A metagenomics method for the quantitative detection of bacterial pathogens causing hospital-associated and ventilator-associated pneumonia.

Microbiology spectrum·2023
Same author

Multicenter Evaluation of the Novel ETEST Fosfomycin for Antimicrobial Susceptibility Testing of Enterobacterales, Enterococcus faecalis, and Staphylococcus Species.

Journal of clinical microbiology·2022
Same author

Evaluation of Tween 80 incorporated media to increase pathogen isolation from peritoneal fluid of CAPD patients at Hospital Kuala Lumpur.

The Malaysian journal of pathology·2021
Same author

Molecular characterization of piezotolerant and stress-resistant mutants of Staphylococcus aureus.

Journal of applied microbiology·2020
Same author

Epidemiological aspects of healthcare-associated infections and microbial genomics.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2018

Related Experiment Video

Updated: Apr 11, 2026

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
09:34

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

2.4K

A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens.

S Asmar1, S Chatellier2, C Mirande2

  • 1Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, Inserm 1095, Marseille, France.

Journal of Clinical Microbiology
|June 5, 2015
PubMed
Summary

A new blood-free medium, MOD9, significantly speeds up the detection of Mycobacterium tuberculosis, the bacteria causing tuberculosis. This advancement improves upon traditional culture methods for faster laboratory diagnosis.

More Related Videos

Growth of Mycobacterium tuberculosis Biofilms
09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

24.6K
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
08:27

Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Published on: October 31, 2019

8.2K

Related Experiment Videos

Last Updated: Apr 11, 2026

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
09:34

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

2.4K
Growth of Mycobacterium tuberculosis Biofilms
09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

24.6K
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
08:27

Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Published on: October 31, 2019

8.2K

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Tuberculosis diagnosis relies on culturing Mycobacterium tuberculosis.
  • Existing culture methods can be time-consuming.
  • There is a need for improved diagnostic media for mycobacteria recovery.

Purpose of the Study:

  • To evaluate the performance of MOD9, a novel blood-free solid medium, for Mycobacterium tuberculosis isolation.
  • To compare the efficacy of MOD9 with the standard Lowenstein-Jensen (LJ) medium.
  • To assess the time to detection and recovery rates of M. tuberculosis using MOD9.

Main Methods:

  • MOD9 medium was tested against Lowenstein-Jensen medium using known M. tuberculosis isolates and clinical specimens.
  • Specimens were decontaminated using chlorhexidine.
  • Key performance indicators included time to colony growth, contamination rates, and isolate recovery.

Main Results:

  • MOD9 demonstrated significantly faster M. tuberculosis growth compared to LJ medium (P < 0.05).
  • MOD9 showed a lower contamination rate (1.6%) than LJ medium (4.4%).
  • MOD9 yielded more M. tuberculosis isolates (15.2%) than LJ medium (12.8%), with eight isolates exclusively recovered from MOD9.

Conclusions:

  • MOD9 is a superior medium to LJ for M. tuberculosis recovery after chlorhexidine decontamination.
  • The use of MOD9 can accelerate the laboratory diagnosis of tuberculosis.
  • MOD9 offers improved efficiency in mycobacterial culture.