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Related Experiment Video

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Implementation of a Reference Interferometer for Nanodetection
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DiameterJ: A validated open source nanofiber diameter measurement tool.

Nathan A Hotaling1, Kapil Bharti2, Haydn Kriel3

  • 1Biosystems & Biomaterials Division, National Institute of Standards & Technology, Gaithersburg, MD, 20899, USA.

Biomaterials
|June 5, 2015
PubMed
Summary
This summary is machine-generated.

A new ImageJ/FIJI plugin, DiameterJ, offers rapid, automated analysis of nanofiber diameter from scanning electron microscope (SEM) images. This free, open-source tool significantly improves accuracy and speed for tissue engineering scaffold analysis.

Keywords:
FIJIImage analysisImageJMorphologyScaffoldStructure

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Image Analysis

Background:

  • Nanofiber scaffolds are crucial for tissue engineering.
  • Current methods for analyzing nanofiber diameter from SEM images are slow, manual, and lack validation.
  • A need exists for a rapid, automated, and accessible solution for nanofiber diameter measurement.

Purpose of the Study:

  • To develop a user-friendly ImageJ/FIJI plugin for rapid, automated nanofiber diameter analysis from SEM micrographs.
  • To ensure compatibility with existing segmentation algorithms and provide open-source code.
  • To validate the plugin's accuracy and speed compared to existing methods and manual assessment.

Main Methods:

  • Development of the "DiameterJ" plugin for ImageJ/FIJI.
  • Integration of centerline determination and Euclidean distance transforms.
  • Implementation of a novel pixel transformation technique.
  • Validation using synthetic images and SEM micrographs of steel wires with known diameters.

Main Results:

  • DiameterJ analyzes SEM micrographs in approximately 20 seconds.
  • The plugin provides measurements statistically indistinguishable from known values.
  • DiameterJ is significantly more accurate and faster than other open-source software and manual measurement.
  • It offers a vastly increased sampling rate compared to manual methods.

Conclusions:

  • DiameterJ provides a validated, rapid, and automated solution for nanofiber diameter analysis.
  • The plugin enhances the study of nanofiber scaffold structural features.
  • It has the potential to advance understanding of fiber diameter distribution and its impact on cell response.