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Related Concept Videos

MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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Profiling circulating microRNAs in maternal serum and plasma.

Qinyu Ge1, Yanting Shen1, Fei Tian1

  • 1Key Lab for Child Development and Learning Science, Ministry of Education, Research Center for Learning Science, Southeast University, Nanjing, Jiangsu 210096, P.R. China.

Molecular Medicine Reports
|June 6, 2015
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Summary

Serum and plasma contain different microRNAs (miRNAs). This study found distinct miRNA profiles in maternal serum versus plasma, suggesting potential for new diagnostic markers in pregnancy.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Serum and plasma are crucial biological fluids for clinical diagnostics.
  • The differential presence and role of nucleic acids, particularly microRNAs (miRNAs), between serum and plasma are not fully understood.
  • Understanding these differences is key for optimizing clinical applications.

Purpose of the Study:

  • To investigate the differential microRNA profiles in maternal serum and plasma.
  • To identify specific microRNAs that are uniquely expressed or differentially abundant between serum and plasma.
  • To assess the potential of these differentially expressed miRNAs as biomarkers in clinical diagnosis.

Main Methods:

  • Next-generation sequencing was employed to profile microRNAs in maternal serum and plasma.
  • Differential expression analysis was performed to identify significant variations.
  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to validate the sequencing findings for selected miRNAs.

Main Results:

  • A total of 329 miRNAs were detected in serum and 193 in plasma.
  • Significant differential expression was observed, with 19 miRNAs upregulated and 6 downregulated in serum compared to plasma (fold change >2.0, P<0.001).
  • Several miRNAs found in serum were absent in plasma, and RT-qPCR confirmed these differential expression patterns.

Conclusions:

  • MicroRNA content differs significantly between maternal serum and plasma.
  • These distinct miRNA profiles suggest potential diagnostic value and warrant further investigation for clinical applications.
  • Differential expression of miRNAs may contribute to the observed differences in DNA and RNA found in serum and plasma.