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Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections

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Heat differentiated complement factor profiling.

Carl Hamsten¹, Lillemor Skattum², Lennart Truedsson²

¹Clinical Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and University Hospital, Stockholm, Sweden; Center for Inflammatory Diseases, Karolinska Institutet, Stockholm, Sweden.

Journal of Proteomics

|June 7, 2015

Summary

This study introduces a new multiplex assay for screening complement deficiencies in newborns. Optimized sample preparation, including heat treatment, improves the detection of crucial complement proteins for better immunodeficiency diagnosis.

Keywords:

Complement factors Dried blood spots Immunodeficiency Multiplex immunoassay Neonatal screening Serum

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Last Updated: Apr 11, 2026

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections

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Measuring the 50% Haemolytic Complement CH50 Activity of Serum

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Published on: March 29, 2010

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

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Published on: January 29, 2014

Area of Science:

Immunology and Proteomics
Innate and Adaptive Immune Responses
Complement System Function

Background:

Complement cascade is vital for innate and adaptive immunity.
Undiagnosed complement deficiencies persist due to limited high-throughput screening tools.
Neonatal screening for immunodeficiencies requires efficient diagnostic methods.

Purpose of the Study:

To develop and validate a multiplex profiling approach for neonatal proteome screening of immunodeficiencies.
To assess the impact of sample preheating on the detectability of nine complement proteins.
To evaluate the feasibility of using dried blood spots for complement component analysis.

Main Methods:

Utilized antibody bead arrays for multiplex profiling of nine complement proteins.
Investigated heat-dependent detectability of complement components in serum and dried blood spots.
Analyzed samples from 16 complement-deficient patients and 23 controls.

Main Results:

Preheating serum samples improved detection of C1q, C2, C3, C6, C9, and factor H.
Heating negatively impacted C7, C8, and factor I detection, requiring non-heated samples.
Demonstrated successful classification of C2-deficiencies using eluates from dried blood spots.

Conclusions:

Multiplexed single-binder assays show significant potential for screening complement deficiencies.
Optimized sample preparation, including heat treatment, enhances diagnostic accuracy.
The developed method facilitates expanded screening for various immunodeficiencies.