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Updated: Apr 11, 2026
Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry
Published on: November 21, 2025
An improvement on mass spectrometry-based epigenetic analysis of large histone-derived peptides by using the
Tetsuya Fukuda1, Hiroshi Hike2, Fumihiko Usui2
1Research and Development, Biosys Technologies, Meguro-Ku, Tokyo 152-0031, Japan.
Abstract:
Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS)-based epigenetic analysis because their relatively low m/z, due to a high degree of proton addition to peptides, would make it difficult to analyze the resulting complex MS/MS spectra. To reduce the degree of protonations, we have developed a new interface, the Ionization Variable Unit (IVU), in which peptides are ionized under a vaporized organic solvent. It is demonstrated that the doubly charged histone tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243, +2), which was not detectable before, can be detected by using the IVU interface and sequenced.
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