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Related Experiment Video

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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
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A knowledge base for Vitis vinifera functional analysis.

Alfredo Pulvirenti, Rosalba Giugno, Rosario Distefano

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    |June 9, 2015
    PubMed
    Summary
    This summary is machine-generated.

    BIOWINE is a new bioinformatics tool for analyzing grapevine (Vitis vinifera) genomes, focusing on Sicilian cultivars. It aids in understanding gene function and microRNA roles under various environmental conditions to improve grape quality and safety.

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    Area of Science:

    • Genomics
    • Bioinformatics
    • Plant Science

    Background:

    • Vitis vinifera (Grapevine) is crucial for global economies, with quality and safety being key concerns in wine production.
    • Genomic approaches are vital for understanding grapevine biology, including fruit maturation and responses to environmental stress.
    • Existing plant genome analysis systems lack specific features for grapevine functional genomics, especially concerning microRNA data and environmental factors.

    Purpose of the Study:

    • To introduce BIOWINE, a novel knowledge base for the functional analysis of Vitis vinifera genomes from Sicilian cultivars.
    • To enable the analysis of RNA-seq experiments for specific cultivars (Nero d'Avola, Nerello Mascalese) under diverse conditions.
    • To provide tools for deeper biological insights into grapevine adaptability and stress responses.

    Main Methods:

    • Development of the BIOWINE knowledge base with a web interface for functional analysis of grapevine genomes.
    • Integration of RNA-seq data analysis for two Sicilian grapevine cultivars.
    • Utilization of a customized GBrowse for genome assembly and RNA-seq data visualization.
    • Implementation of gene set enrichment analysis using third-party databases.

    Main Results:

    • BIOWINE provides a platform for analyzing grapevine genomes and RNA-seq data.
    • The system supports the investigation of gene and microRNA functions in relation to environmental factors.
    • Users can explore gene set enrichment to understand biological pathways.

    Conclusions:

    • BIOWINE is a valuable bioinformatics resource for grapevine genome analysis.
    • The knowledge base enhances understanding of Sicilian grapevine varieties' adaptability to climate, disease, and location.
    • BIOWINE facilitates research into improving grape quality and safety through genomic insights.