Related Experiment Video
Updated: Apr 11, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
Published on: December 6, 2013
A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production
Nicholas J Mozdzierz1, Kerry R Love, Kevin S Lee
1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. clove@mit.edu.
Abstract:
We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.
Related Concept Videos
Upstream Processing
Bioreactor Controls-II

