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Published on: January 5, 2021
Amino acids profile of sugar cane spirit (cachaça), rum, and whisky
Francisco W B Aquino1, Lisangela M Boso1, Daniel R Cardoso1
1Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São Carlense 400, CP 780, CEP 13560-970, São Carlos - SP, Brazil.
Abstract:
An analytical procedure for the separation and quantification of 20 amino acids in cachaças has been developed involving C18 solid phase cleanup, derivatization with o-phthalaldehyde/2-mercaptoethanol, and reverse phase liquid chromatography with fluorescence detection. The detection limit was between 0.0050 (Cys) and 0.25 (Ser)mgL(-1), whereas the recovery index varies from 69.5 (Lys) to 100 (Tyr)%. Relative standard deviations vary from 1.39 (Trp) to 13.4 (Glu)% and from 3.08 (Glu) to 13.5 (His) for the repeatability and intermediate precision, respectively. From the quantitative profile of amino acids in 41 cachaças, 5 rums, and 12 whisky samples, the following order of amino acids in significant quantities is observed: Gly=Ser
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