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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.7K

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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
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[Atomic force microscopy: a tool to analyze the viral cycle].

Julien Bernaud1, Martin Castelnovo1, Delphine Muriaux2

  • 1Laboratoire de physique, CNRS UMR 5672, Ecole normale supérieure de Lyon, 46, allée d'Italie, 69364 Lyon Cedex 07, France.

Medecine Sciences : M/S
|June 11, 2015
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Summary

Atomic force microscopy (AFM) reveals physical changes in HIV-1 during its life cycle. This technique visualizes viral capsids and measures protein-receptor interactions at the single-molecule level.

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Area of Science:

  • Virology
  • Biophysics
  • Nanotechnology

Background:

  • The human immunodeficiency virus type 1 (HIV-1) life cycle involves dynamic alterations in viral particle morphology and mechanics.
  • Understanding these physical changes is crucial for developing effective antiviral strategies.

Purpose of the Study:

  • To demonstrate the utility of Atomic Force Microscopy (AFM) in characterizing physical changes in HIV-1 at the single-virus level.
  • To explore the application of AFM for probing viral capsid mechanics and molecular interactions.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) for high-resolution imaging of viral capsids in physiological conditions.
  • Employing AFM-based nano-indentation to measure the mechanical properties of individual viral particles.
  • Applying AFM force spectroscopy to quantify the binding affinities between HIV-1 envelope proteins and cellular receptors.

Main Results:

  • AFM successfully visualized HIV-1 capsids, providing insights into their structural integrity.
  • Nano-indentation experiments revealed changes in the mechanical properties of the viral core during different life cycle stages.
  • Force spectroscopy accurately characterized single-molecule interactions between viral proteins and receptors.

Conclusions:

  • AFM is a powerful tool for investigating the physical properties of HIV-1 at the nanoscale.
  • AFM-based techniques offer novel approaches to study viral dynamics and molecular recognition events relevant to HIV-1 infection.