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Related Experiment Videos

Mineral induction by polyanionic dentin and bone proteins at physiological ionic conditions.

A Linde1, A Lussi

  • 1Department of Oral Biochemistry, Gothenburg University, Sweden.

Connective Tissue Research
|January 1, 1989
PubMed
Summary

Immobilized polyanionic proteins from bone and dentin, like phosphoprotein and proteoglycan, can initiate apatite formation. This occurs even though these macromolecules typically inhibit mineral growth when in solution.

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Area of Science:

  • Biomineralization
  • Biochemistry
  • Materials Science

Background:

  • Polyanionic proteins in calcified tissues are thought to initiate mineral formation by binding calcium ions.
  • However, these proteins in solution are known to inhibit apatite induction and growth.

Purpose of the Study:

  • To investigate the role of immobilized non-collagenous macromolecules in initiating apatite formation.
  • To determine if immobilized bone and dentin macromolecules can induce apatite under physiological conditions.

Main Methods:

  • Review of studies examining the effects of immobilized non-collagenous macromolecules on apatite formation.
  • In vitro experiments assessing apatite induction by immobilized phosphoprotein and proteoglycan at physiological ion concentrations.

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Main Results:

  • Minute amounts of immobilized non-collagenous macromolecules from dentin and bone, including phosphoprotein and proteoglycan, were shown to induce apatite.
  • This induction occurred at physiological calcium and phosphate ion concentrations.

Conclusions:

  • Immobilization is a critical factor for the ability of polyanionic proteins to initiate biomineralization.
  • Non-collagenous macromolecules play a key role in initiating apatite formation in calcified tissues when properly localized.