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SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients
Ulla Rüetschi1, Martin Stenson2, Sverker Hasselblom3
1Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden ; Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden.
International Journal of Proteomics
|June 11, 2015
Summary
Diffuse large B-cell lymphoma (DLBCL) patients who responded well to treatment showed higher expression of actin cytoskeleton proteins. This finding suggests a potential role for these proteins in sustained response to immunochemotherapy.
Area of Science:
- Proteomics
- Oncology
- Cell Biology
Background:
- Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma.
- Early relapse or refractory disease in DLBCL has a poor prognosis despite immunochemotherapy.
Purpose of the Study:
- To investigate global protein expression differences between DLBCL patients with distinct clinical outcomes.
- To identify protein network patterns associated with sustained response to immunochemotherapy.
Main Methods:
- Stable Isotope Labeling by Amino acids in Cell culture (SILAC)-based shotgun quantitative proteomic analysis (LC-MS/MS).
- Analysis of fresh-frozen tumor tissue from early relapsed/refractory and long-term progression-free DLBCL patients.
- Functional annotation and supervised regression analysis of differentially expressed proteins.
Main Results:
- Over 3,500 proteins identified; more than 1,300 quantified across all patients.
- 87 proteins were significantly differentially expressed between patient groups.
- Proteins involved in actin cytoskeleton regulation and organization were overexpressed in progression-free patients.
- Five actin cytoskeleton-related proteins could discriminate between the two patient groups.
Conclusions:
- SILAC-based quantitative proteomic analysis is effective for DLBCL proteome exploration.
- Overexpression of actin cytoskeleton proteins correlates with sustained response to immunochemotherapy in DLBCL.
- Actin cytoskeleton protein network patterns may play a functional role in treatment response.
