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Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level
A H Okholm1, H Aslan, F Besenbacher
1Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, 8000 Aarhus C, Denmark. kjems@inano.au.dk.
Nanoscale
|June 11, 2015
Abstract:
DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

