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Updated: Apr 10, 2026
Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain
Yuling Wang1, Eugene J H Wee, Matt Trau
1Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), QLD 4072, Australia. y.wang27@uq.edu.au m.trau@uq.edu.au.
Abstract:
Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.
