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Osteopontin can decrease the cartilage cellular inflammatory reaction induced by LPS
Fengsheng Li1, Dongping Ye1, Jun Duan1
1Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou 510220, P. R. China.
Objective:
This study aims to explore the effect of osteopontin on the inflammatory reaction of cartilage cells stimulated by LPS and its possible signal pathway.
Methods:
The inflammatory reaction of cartilage cells was induced by LPS, and then the cells were treated with OPN and PD98059 respectively. The expression of TNF-α, IL-1β and ERK1/2 were detected by ELISA or Western-blotting methods.
Results:
Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS with dose dependent, while PD98059 could reverse the inhibition of osteopontin.
Conclusion:
Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS, which may be associated with the ERK1/2 signal pathway.
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