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Related Experiment Video

Updated: Apr 10, 2026

SpOT the Correct Tissue Every Time in Multi-tissue Blocks
06:53

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

12.5K

SpOT the Correct Tissue Every Time in Multi-tissue Blocks.

Anna Coffey1, Michael D Johnson2, Deborah L Berry3

  • 1Center for Advanced Preclinical Research (CAPR), Frederick National Laboratory for Cancer Research; Leidos Biomedical Research, Inc.

Journal of Visualized Experiments : Jove
|June 13, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed the Specimen Orientation Tag (SpOT) for multi-tissue paraffin blocks. This simple tool ensures reliable tissue identification in both arrayed and non-arrayed formats, enhancing high-throughput analysis.

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Area of Science:

  • Histology and Pathology
  • Biotechnology
  • Laboratory Science

Background:

  • Multi-tissue paraffin blocks enable high-throughput analysis, improving efficiency and reducing costs.
  • While tissue microarrays are common, non-arrayed blocks with larger tissues offer similar benefits with less investment.
  • Accurate tissue orientation is crucial for multi-tissue analysis but existing methods are often unsuitable for non-arrayed blocks.

Purpose of the Study:

  • To introduce a novel, cost-effective orientation tool for multi-tissue paraffin blocks.
  • To address the limitations of current orientation methods in non-arrayed block formats.
  • To ensure reliable specimen identification in both arrayed and non-arrayed multi-tissue block layouts.

Main Methods:

  • Development of the Specimen Orientation Tag (SpOT), a simple, low-cost orientation tool.
  • Evaluation of SpOT's visibility in paraffin blocks and subsequent tissue sections.
  • Assessment of SpOT's compatibility with both arrayed and non-arrayed tissue arrangements.

Main Results:

  • The Specimen Orientation Tag (SpOT) is clearly visible in paraffin blocks and all tissue sections.
  • SpOT facilitates reliable specimen identification in both arrayed and non-arrayed multi-tissue blocks.
  • SpOT does not require direct tissue modification and allows flexible tissue arrangement.

Conclusions:

  • The Specimen Orientation Tag (SpOT) is an effective and versatile tool for multi-tissue block orientation.
  • SpOT overcomes limitations of existing methods, particularly for non-arrayed block formats.
  • This tool enhances the efficiency and reliability of high-throughput histological analysis.