Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture
Célio Fernando F Angolini1, Pedro Henrique Vendramini1, Francisca D S Araújo1
1†Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
Abstract:
Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.
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