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Profiling of Oral and Nasal Microbiome in Children With Cleft Palate

Min Zhang, Renke Wang, Ying Liao

    The Cleft Palate-Craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association
    |June 13, 2015
    PubMed

    Insights

    Children with cleft palate have altered oral and nasal microbiomes, with reduced diversity and different bacterial compositions. This oral microbiome disturbance may link to increased infectious disease risk.

    Area of Science:

    • Microbiology
    • Genetics
    • Pediatrics

    Background:

    • Cleft palate is associated with a higher prevalence of infectious diseases.
    • The oral and nasal microbial profiles in children with cleft palate are not well understood.

    Purpose of the Study:

    • To identify the oral and nasal microbial profiles in children with cleft palate.
    • To compare these profiles with those of healthy children.
    • To explore potential links between the microbiome and infectious diseases.

    Main Methods:

    • Analysis of saliva and nasal samples from 10 cleft palate children and 10 age-matched controls.
    • DNA isolation, PCR-DGGE for microbial fingerprinting, and DNA sequencing.

    Main Results:

    • Significantly lower saliva microbial diversity in cleft palate children.
    • Distinct microbial compositions in saliva and nares of cleft palate children.
    • Increased oral-nasal microbial similarity in cleft palate children.
    • Reduced presence of Lautropia and Bacillus species in cleft palate children's saliva.
    • Fewer Dolosigranulum and Bacillus species in cleft palate children's nasal cavities.
    • Predominance of Streptococcus species in the nasal cavities of cleft palate children.

    Conclusions:

    • Children with cleft palate exhibit a disturbed oral and nasal microbiome.
    • Abnormal communication between oral and nasal cavities may contribute to microbiome alterations.
    • Further research is needed to establish the relationship between the disturbed microbiome and disease.
    Abstract

    Keywords:
    16S rRNADGGEcleft palatemicrobiome diversity

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