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Updated: Apr 10, 2026

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Published on: November 19, 2020
Establishment and Use of Mouse Haploid ES Cells
Martin Leeb1, Anthony C F Perry2, Anton Wutz3
1Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom.
None:
Haploid genetics has facilitated new insights into mammalian pathways and disease mechanisms. Most animal cells are diploid, and mammalian haploid cell cultures have remained elusive for a long time. Recent methodological progress has enabled the routine derivation of haploid stem cell lines from mammalian haploid embryos. Here we provide detailed protocols for the establishment, culture, and manipulation of parthenogenetic and androgenetic haploid embryonic stem cells from mouse embryos.

