Enhanced angiogenesis of growth factor-free porous biodegradable adhesive made with hexanoyl group-modified gelatin

The bonding behavior of hexanoyl (Hx: C6) group-modified alkaline-treated gelatin (HxAlGltn) porous films ((P)HxAlGltn) on the porcine intestine was evaluated. (P)HxAlGltns with various porosities were prepared by the salt-leaching method for various solid-liquid ratios. (P)HxAlGltns bonded more strongly to porcine intestine surfaces than did porous AlGltn films ((P)AlGltns). L929 cells cultured on (P)HxAlGltns showed adhesivity than cells cultured on (P)AlGltns. Faster tissue infiltration and a shorter degradation time of highly porous (P)HxAlGltns were observed after implantation in rat subcutaneous tissues. The angiogenic markers CD34 and α-SMA were highly expressed around (P)HxAlGltns that had high porosity. These results indicated that highly porous (P)HxAlGltns have advantages with respect to not only bonding strength on wet soft tissues, but also angiogenesis.