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Updated: Apr 10, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Interactions of Isophorone Derivatives with DNA: Spectroscopic Studies
Marco Deiana1, Katarzyna Matczyszyn1, Julien Massin2
1Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370, Wroclaw, Poland.
Abstract:
Interactions of three new isophorone derivatives, Isoa Isob and Isoc with salmon testes DNA have been investigated using UV-Vis, fluorescence and circular dichroism spectroscopic methods. All the studied compounds interact with DNA through intercalative binding mode. The stoichiometry of the isophorone/DNA adducts was found to be 1:1. The fluorescence quenching data revealed a binding interaction with the base pairs of DNA. The CD data indicate that all the investigated isophorones induce DNA modifications.
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