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Flow cytometric methods for the analysis of human basophil surface antigens and viability

B S Bochner1, A A McKelvey, R P Schleimer

  • 1Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21239.

Journal of Immunological Methods
|December 20, 1989
PubMed

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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