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Genetic relationships among Enterococcus faecalis isolates from different sources as revealed by multilocus sequence

X Chen1, Y Q Song1, H Y Xu1

  • 1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China.

Journal of Dairy Science
|June 16, 2015
PubMed
Summary

Genetic diversity of Enterococcus faecalis was analyzed using multilocus sequence typing. This study reveals distinct lineages and high recombination rates in E. faecalis from food and human sources.

Keywords:
Enterococcus faecalisgenetic relationshipmultilocus sequence typing

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Area of Science:

  • Microbiology
  • Genetics
  • Food Science

Background:

  • Enterococcus faecalis is a common gut bacterium with food production applications.
  • Understanding the genetic diversity and phylogenetic relationships of E. faecalis from various sources is crucial.

Purpose of the Study:

  • To evaluate the genetic diversity and phylogenetic relationships among Enterococcus faecalis isolates from Chinese food products and other sources.
  • To establish a sharable database for global strain comparison and advance understanding of E. faecalis microbial ecology.

Main Methods:

  • Multilocus sequence typing (MLST) protocol was employed.
  • 39 E. faecalis isolates from Chinese traditional foods (dairy, acidic gruel) and 4 published isolates (human-derived) were analyzed.
  • Five housekeeping genes (groEL, clpX, recA, rpoB, pepC) were utilized.

Main Results:

  • Twenty-three unique sequence types were identified, forming 5 clonal complexes and 10 singletons.
  • A high frequency of intraspecies recombination was indicated by the standardized index of association (IA(S)=0.1465) and network structure.
  • Enterococcus faecalis lineages showed source-clustered distributions, with dairy isolates grouping together and acidic gruel isolates showing close relation to a human isolate.

Conclusions:

  • The MLST scheme provides a valuable, continuously growing database for global Enterococcus faecalis strain comparison.
  • The findings advance the understanding of the microbial ecology and population structure of Enterococcus faecalis across different environments.