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Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection.
Anurag Sharma1, Elizabeth Clark1, James D McGlothlin2
1Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette IN, USA.
Frontiers in Microbiology
|June 16, 2015
Summary
The Airborne Sample Analysis Platform (ASAP) 2800 effectively collects and detects airborne viruses. This bioaerosol sampler shows promise for rapid pathogen detection in environmental monitoring.
Area of Science:
- Environmental Science
- Microbiology
- Public Health
Background:
- Bioterrorism and pandemics necessitate rapid bioaerosol detection.
- Continuous air sampling is crucial for pathogen surveillance.
- Existing methods require improvement for real-time threat assessment.
Purpose of the Study:
- To evaluate the Airborne Sample Analysis Platform (ASAP) 2800 for bioaerosol sampling.
- To assess the platform's efficacy in detecting airborne viruses.
- To validate the ASAP for pathogen detection in environmental air.
Main Methods:
- Aerosolization of a surrogate virus (bovine adenovirus serotype 3).
- Collection of aerosolized virus using the ASAP 2800 bioaerosol sampler.
- Detection of the collected virus using quantitative PCR (qPCR).
Main Results:
- The ASAP 2800 efficiently trapped the surrogate virus at concentrations of 5 × 10(3) p.f.u. or higher.
- Successful detection of the aerosolized virus was achieved via qPCR.
- The platform demonstrated capability in collecting representative air samples for virus identification.
Conclusions:
- The ASAP 2800 is effective for collecting and detecting airborne viruses.
- This technology supports the development of advanced bioaerosol pathogen detection systems.
- ASAP shows potential for safeguarding public health against bioterrorism and pandemics.
