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Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

Atomic Fluorescence Spectroscopy

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...

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Related Experiment Video

Updated: Apr 10, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Narrow-Band Emitting Solid Fluorescence Reference Standard with Certified Intensity Pattern.

Katrin Hoffmann¹, Monika Spieles¹, Wolfram Bremser¹

¹BAM Federal Institute for Materials Research and Testing, Division 1.10, Richard-Willstaetter-Strasse 11, D-12489 Berlin, Germany.

Analytical Chemistry

|June 17, 2015

Summary

This summary is machine-generated.

A new lanthanum-phosphate glass doped with rare-earth ions serves as a solid fluorescence standard. This reference material validates fluorescence measuring devices with its stable emission spectrum.

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Last Updated: Apr 10, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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Published on: April 27, 2018

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Published on: August 22, 2019

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

Area of Science:

Materials Science
Spectroscopy
Analytical Chemistry

Background:

Fluorescence measurements require reliable standards for accurate performance validation.
Existing fluorescence standards may have limitations in stability or spectral characteristics.

Purpose of the Study:

To develop and characterize a novel solid fluorescence standard material.
To provide a reliable tool for day-to-day performance validation of fluorescence measuring devices.

Main Methods:

Development of lanthanum-phosphate glass doped with rare-earth ions.
Characterization of optical properties including absorption spectra, fluorescence anisotropy, and temperature dependence.
Evaluation of homogeneity, photo-stability, and long-term stability.
Establishment of a certification procedure with normalized intensity profiles and uncertainty calculations.

Main Results:

The developed glass exhibits a characteristic emission intensity pattern between 450-700 nm upon 365 nm excitation.
Comprehensive characterization confirmed properties relevant to its use as a standard.
A certification procedure was successfully developed and implemented for the reference material BAM-F012.

Conclusions:

The novel rare-earth-doped glass is a promising solid fluorescence standard.
The material offers a ready-to-use solution for validating fluorescence instruments.
The developed characterization and certification methods ensure reliable performance data.