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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
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Transcriptome modulations due to A/C2 plasmid acquisition.

Kevin S Lang1, Timothy J Johnson1

  • 1Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.

Plasmid
|June 17, 2015
PubMed
Summary
This summary is machine-generated.

Bacterial plasmids drive genetic diversity and spread antimicrobial resistance. This study found that a plasmid

Keywords:
A/C2CrosstalkGene expressionHGTRNA-Seq

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Area of Science:

  • Bacteriology
  • Genetics
  • Molecular Biology

Background:

  • Plasmids contribute to bacterial genetic diversity and the spread of antimicrobial resistance.
  • Epistatic interactions between host chromosomes and plasmids influence plasmid fitness, but these interactions are host-specific.

Purpose of the Study:

  • To investigate how different bacterial host backgrounds affect the transcriptome of the A/C2 plasmid.
  • To understand the impact of plasmid acquisition on host chromosomal gene expression.

Main Methods:

  • RNA-Sequencing (RNA-Seq) was employed to analyze transcriptomes.
  • Experiments were conducted across six bacterial strains from three species.
  • Chromosomal transcriptomes were compared in the presence and absence of the A/C2 plasmid pAR060302 in five strains.

Main Results:

  • Host background significantly influences A/C2 plasmid gene expression.
  • Acquisition of plasmid pAR060302 altered the expression of host chromosomal genes related to metabolism and energy production.
  • Host-specific effects on both plasmid and chromosomal gene expression were identified.

Conclusions:

  • Plasmid fitness is influenced by host chromosome content.
  • Environmental factors also play a role in plasmid persistence and fitness.
  • Understanding host-plasmid interactions is crucial for managing antimicrobial resistance spread.