RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae
Yuko Imamura1, Feifei Yu1, Misaki Nakamura1
1Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8530, Japan.
Plos One
|June 19, 2015
Summary
The Remodel the Structure of Chromatin (RSC) complex is vital for yeast growth and mitochondrial function. RSC isoforms play redundant roles in respiratory growth and share target genes with the HAP complex.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Mitochondrial Biology
Background:
- The Remodel the Structure of Chromatin (RSC) complex is an ATP-dependent complex crucial for Saccharomyces cerevisiae growth.
- RSC exists in two isoforms, differing in Rsc1 or Rsc2 subunits, but sharing core components like the ATPase Nps1/Sth1.
Purpose of the Study:
- To investigate the connection between RSC function and mitochondrial respiration.
- To elucidate the roles of RSC isoforms in yeast growth and gene regulation.
Main Methods:
- Synthetic Genetic Array (SGA) analysis using a temperature-sensitive nps1-105 mutant.
- Phenotypic analysis of rsc mutants, including respiratory growth assays and mitochondrial morphology assessment.
- Genome-wide expression analysis and protein-protein interaction studies.
Main Results:
- RSC mutants (rsc1Δ, rsc2Δ, nps1-13) displayed defective respiratory growth and mitochondrial aggregation.
- RSC1 overexpression rescued rsc2Δ phenotypes, indicating isoform redundancy in respiratory growth.
- RSC regulates transcription of HAP complex target genes, and Nps1 interacts with Hap4.
Conclusions:
- RSC plays critical roles in mitochondrial gene expression and respiratory function in yeast.
- RSC isoforms exhibit functional redundancy, particularly in maintaining respiratory growth.
- RSC and the HAP complex cooperate in regulating a shared set of genes involved in mitochondrial function.
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