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Related Experiment Video

Updated: Jan 20, 2026

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
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Quantifying the chronic effect of low DO on the nitrification process.

Guoqiang Liu1, Jianmin Wang2

  • 1Department of Civil, Architectural and Environmental Engineering, Missouri University of Science and Technology, Rolla, MO 65409, United States; Frontier Environmental Technology, 12687 Cinnamon Court, Rolla, MO 65401, United States.

Chemosphere
|June 19, 2015
PubMed
Summary

Low dissolved oxygen (DO) enriches nitrifying bacteria, enabling complete nitrification. This study quantifies DO

Keywords:
Half-velocity constantLow DO aerationModelingNitrification kineticsNitrifier decayNitrifier growth

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Area of Science:

  • Environmental Microbiology
  • Wastewater Treatment Engineering
  • Biogeochemical Cycles

Background:

  • Previous research indicated low dissolved oxygen (DO) enriches nitrifier communities, enabling complete nitrification.
  • Understanding the chronic effects of low DO on nitrification kinetics is crucial for optimizing wastewater treatment.

Purpose of the Study:

  • To determine nitrifier kinetic constants under low DO conditions.
  • To quantify the chronic effect of low DO on ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB).
  • To establish a theoretical foundation for low-DO operation in advanced wastewater treatment plants for energy efficiency.

Main Methods:

  • Determination of nitrifier kinetic constants (half-velocity constants for growth and decay) for AOB and NOB.
  • Quantification of the chronic effects of low DO on the nitrification process.
  • Development of a relationship between operational DO and required Solids Retention Time (SRT) for complete nitrification.

Main Results:

  • Half-velocity constants for DO on AOB growth (KDO-g) and decay (KDO-d) were 0.29 and 0.48 mg/L.
  • Half-velocity constants for DO on NOB growth (KDO-g) and decay (KDO-d) were 0.08 and 0.69 mg/L.
  • Low DO (<1 mg/L) inhibits AOB and NOB decay, leading to enrichment, while DO >1 mg/L offers no additional nitrification benefit.

Conclusions:

  • Nitrification is feasible under long-term low DO conditions due to inhibited decay of AOB and NOB.
  • Nitrite oxidation by NOB is less impacted by low DO compared to ammonia oxidation by AOB.
  • Optimizing DO levels in advanced wastewater treatment can significantly improve aeration energy efficiency.