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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Related Experiment Video

Updated: Apr 9, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

LOV-based reporters for fluorescence imaging.

Anthony M Buckley¹, Jan Petersen², Andrew J Roe¹

¹Institute of Infection, Immunology & Inflammation, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.

Current Opinion in Chemical Biology

|June 19, 2015

Summary

New fluorescent proteins (FPs) derived from plant and bacterial LOV (light, oxygen, or voltage sensing) domains offer advantages over GFP. These stable, small FPs show promise for bioimaging and photodynamic therapy applications.

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Last Updated: Apr 9, 2026

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Genetically encoded fluorescent proteins (FPs) are crucial tools in biological research.
Chromophore-binding domains from photoreceptors are emerging as novel FP sources.
Flavin-binding LOV (light, oxygen, or voltage sensing) domains are a promising class of these new FPs.

Purpose of the Study:

To review the potential applications of LOV-based fluorescent proteins.
To discuss the advantages and limitations of LOV-based FPs compared to traditional FPs like GFP.
To provide insights into the future development of LOV FP technology for bioimaging and photodynamic therapy.

Main Methods:

Literature review of recent studies on LOV-based FPs.
Comparative analysis of LOV FPs and green fluorescent protein (GFP).
Discussion of technological advancements and future directions.

Main Results:

LOV-based FPs exhibit advantages such as smaller size, enhanced pH and thermal stability, and utility under anaerobic conditions.
These FPs can also generate reactive oxygen species, opening avenues for specific applications.
LOV FPs present a viable alternative to GFP with unique benefits.

Conclusions:

LOV-based FPs represent a significant advancement in fluorescent reporter technology.
Their unique properties make them highly suitable for advanced bioimaging techniques.
Further development holds great potential for applications in photodynamic therapy and beyond.