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Related Experiment Video

Updated: Apr 9, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

A Shortcut to Activity-Dependent Transcription.

Nikhil Sharma¹, Harrison W Gabel¹, Michael E Greenberg¹

¹Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.

|June 20, 2015

Summary

Researchers discovered that topoisomerase IIB (an enzyme) unexpectedly cuts promoter DNA. This cutting action facilitates the transcription of genes activated by neuronal activity.

Area of Science:

Molecular biology
Neuroscience
Genetics

Background:

Neuronal activity triggers rapid gene transcription via molecular pathways.
Gene expression regulation is crucial for neuronal function and adaptation.

Purpose of the Study:

To investigate the molecular mechanisms underlying activity-induced gene transcription.
To identify novel factors involved in regulating gene expression in response to neuronal stimulation.

Main Methods:

The study involved analyzing DNA cutting patterns in promoter regions.
Investigated the role of topoisomerase IIB in gene transcription.
Utilized molecular biology techniques to assess gene expression changes.

Main Results:

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Last Updated: Apr 9, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

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Published on: March 7, 2018

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

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Published on: November 26, 2013

Topoisomerase IIB was found to directly cut promoter DNA.
This DNA cutting by topoisomerase IIB is essential for transcribing activity-induced genes.
Identified a novel function for topoisomerase IIB in gene regulation.

Conclusions:

Topoisomerase IIB plays a critical, previously unrecognized role in facilitating gene transcription.
The cutting of promoter DNA by topoisomerase IIB is a key step in the response to neuronal activity.
This finding opens new avenues for understanding gene regulation in the nervous system.