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Published on: August 3, 2012
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Spatiotemporal expression of Rhomboid domain containing 2 (Rhbdd2) during rat development
V A Ferretti1, R Canzoneri1, C G Barbeito2
1CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
Acta Histochemica
|June 22, 2015
Summary
Rhomboid domain-containing protein 2 (RHBDD2) is highly expressed in embryonic and adult rat tissues, particularly in rapidly dividing cells. This suggests RHBDD2 plays a crucial role in cell proliferation across various life stages.
Area of Science:
- Molecular Biology
- Developmental Biology
- Cancer Research
Background:
- Rhomboid gene family members are implicated in cancer and neurodegenerative diseases.
- Human RHBDD2 is overexpressed in advanced breast and colorectal cancers, suggesting a role in cell proliferation.
- Limited knowledge exists regarding RHBDD2 expression in diverse tissues and species.
Purpose of the Study:
- To investigate the expression patterns of Rhbdd2 in embryonic and adult rat tissues.
- To explore the potential role of Rhbdd2 in cell proliferation, drawing parallels between cancer and embryonic cells.
Main Methods:
- Immunohistochemistry (IHC) to visualize Rhbdd2 protein expression.
- Reverse Transcription Polymerase Chain Reaction (RT-PCR) to quantify Rhbdd2 gene expression.
- Analysis of Rhbdd2 expression in various embryonic and adult rat tissues, including the mammary gland.
- Investigation of Rhbdd2 localization in the mouse mammary epithelial cell line HC11.
Main Results:
- Rvbdd2 was detected in early developmental stages across most analyzed rat tissues, with notable high expression in the brain, spinal cord, kidney, and embryonic skin.
- In adult rats, Rhbdd2 expression remained elevated, with salivary glands also becoming positive.
- Rvbdd2 exhibited high expression during proliferative stages of the rat mammary gland.
- Similar expression patterns were observed in the mouse mammary epithelial cell line HC11 and during mouse mammary gland development, with Rhbdd2 localized to the Golgi apparatus.
Conclusions:
- Rvbdd2 is expressed in various embryonic and adult rat tissues.
- Rvbdd2 expression is particularly high in proliferative tissues, including the mammary gland during development.
- These findings suggest Rhbdd2 is involved in both embryonic and adult tissue proliferation.
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