Affinity Purification of Protein Complexes Using TAP Tags

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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
10:36

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

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Erica Gerace1, Danesh Moazed1

  • 1Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.

Methods in Enzymology
|June 23, 2015
PubMed

Keywords:
Affinity purificationCalmodulin binding (CAM-B)Calmodulin elution (CAM-E)IgG-SepharoseLysis bufferProtease cleavageProtein lysatesTAP tagsTEV cleavage (TEV-C)

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