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Related Experiment Video

Updated: Apr 9, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

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Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation.

Akiyuki Hasegawa1, Yuji Haraguchi1, Tatsuya Shimizu1

  • 1Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.

Journal of Biomedical Materials Research. Part A
|June 23, 2015
PubMed
Summary

Centrifugation significantly speeds up the creation of three-dimensional (3D) tissues using cell sheet technology. This rapid fabrication method reduces manipulation time by two-thirds without compromising cell viability or function.

Keywords:
cell sheet technologycentrifugationoptical coherence tomographyrapid attachmentthree-dimensional tissue

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Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Three-dimensional (3D) tissue reconstruction using cell sheet technology is crucial for tissue regeneration.
  • Conventional cell sheet layering requires 20-30 minutes for adequate cell adhesion.

Purpose of the Study:

  • To develop a faster method for fabricating bioengineered tissues using cell sheet technology.
  • To reduce the time required for cell sheet attachment during tissue construction.

Main Methods:

  • Utilized centrifugation (12-34 x g for 3 min) to accelerate cell sheet attachment.
  • Employed C2C12 mouse myoblast sheets harvested from temperature-responsive culture dishes.
  • Confirmed rapid attachment using optical coherence tomography.

Main Results:

  • Centrifugation reduced cell sheet attachment time from 20-30 minutes to 3 minutes.
  • Rapidly constructed tissues exhibited active cell metabolism, high viability, and significant vascular endothelial growth factor production.
  • No cell damage was observed, indicating complete cell sheet attachment.

Conclusions:

  • Centrifugation offers a rapid and effective method for cell sheet-based tissue engineering.
  • This accelerated system can significantly advance clinical applications in regenerative medicine.
  • The technique ensures tissue integrity and functionality comparable to conventional methods.