Decalcifying capability of irrigating solutions on root canal dentin mineral content

Chelating agents are believed to aid root canal irrigation and to be able to remove the inorganic smear layer.

The purpose of the present study was to evaluate and to compare the decalcifying capability of different irrigating solutions (Tubuliclean, Largal Ultra, ethylenediaminetetraacetic acid 17%, Tetraclean, Tetraclean NA).

Sixty maxillary central incisors were used. Root canals were instrumented and irrigated. From each root, four comparable slices of cervical dentin were obtained. At three successive 5-min interval immersion times, the concentration of calcium extracted from root canal dentin was assessed with an inductively coupled plasma-atomic emission spectrometer.

Data were analyzed by means of Kruskal Wallis and Mann-Whitney tests. Significance was predetermined at P < 0.05.

For all irrigating solutions, the maximum amount of Ca(2+) extracted from root canal dentin samples was reached after 10 min contact time except for citric acid-based agents (Tetraclean and Tetraclean NA) which induced a higher and still increasing calcium release even after 10 min contact time. In order to obtain an efficient decalcifying action on dentin and to facilitate the biomechanical procedures, citric acid-based irrigants can be applied.