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Membrane fluidity changes in polymorphonuclear leukocytes and erythrocytes in psoriasis.
A M Offidani1, O Simonetti, G Ferretti
1Institute of Dermatology, School of Medicine, University of Ancona, Italy.
Acta Dermato-Venereologica. Supplementum
|January 1, 1989
Summary
Psoriatic patients show reduced membrane fluidity in erythrocytes and polymorphonuclear leukocytes. This suggests potential membrane-cytoskeleton interaction defects in psoriasis, impacting cells beyond the skin.
Area of Science:
- Cell Biology
- Dermatology
- Biochemistry
Background:
- Psoriasis is a chronic inflammatory skin condition.
- Altered cell membrane properties may contribute to psoriasis pathogenesis.
- Investigating membrane fluidity offers insights into cellular dysfunction.
Purpose of the Study:
- To investigate membrane fluidity in erythrocytes and polymorphonuclear leukocytes of psoriatic patients.
- To explore potential alterations in membrane lipid composition.
- To identify cellular mechanisms contributing to psoriasis.
Main Methods:
- Utilized fluorescence polarization with 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene.
- Analyzed membrane fluidity in living polymorphonuclear leukocytes.
- Examined erythrocyte membranes from 8 psoriatic patients and controls.
Main Results:
- Observed decreased membrane fluidity in erythrocytes of psoriatic patients.
- Found no significant changes in the cholesterol to phospholipid molar ratio in erythrocyte membranes.
- Detected reduced membrane fluidity in polymorphonuclear leukocytes of psoriatic patients.
Conclusions:
- Psoriasis is associated with altered membrane fluidity in non-epidermal cells.
- Suggests a potential defect in membrane-cytoskeleton interactions in psoriasis.
- Highlights the systemic nature of cellular changes in psoriasis.