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The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects
Published on: October 2, 2014
Comparison of microporous polysaccharide hemospheres and Ankaferd Blood Stopper in a rabbit epistaxis model
Veysel Yurttas1, Murat Sereflican2, Elçin Hakan Terzi3
1Department of Otorhinolaryngology and Head and Neck Surgery, Abant İzzet Baysal University Medical Faculty, Golkoy, 14280, Bolu, Turkey. vyurttas@hotmail.com.
Abstract:
The aim of this study was to evaluate the histopathological impact, effectiveness, and safety of two hemostatic agents, Ankaferd Blood Stopper (ABS) and microporous polysaccharide hemospheres (MPH), in an experimental rabbit epistaxis model. Rabbits were randomly assigned, using a computerized random number generator, to the following three groups of six animals: group 1 (control, irrigated with saline); group 2 (ABS-treated); and group 3 (MPH-treated). In all groups, a standardized rabbit epistaxis model was used. Hemostasis time and extent of nasal bleeding were measured to compare the hemostatic effect of ABS and MPH among groups. Septums were removed for histopathological analysis, 7 days after the procedure. ABS reduced hemostasis time to 104.2 s and amount of bleeding to 20.5 mg. MPH reduced hemostasis time to 71.7 s and amount of bleeding to 11.5 mg. Mean bleeding time in wounds administered ABS and MPH was significantly shorter compared with wounds administered isotonic saline solution (p = 0.004). ABS and MPH application decreased bleeding significantly compared with the control group (p = 0.004). Bleeding time and amount in the MPH group was significantly reduced compared with the ABS group (p = 0.013 and p = 0.004, respectively). There was no significant difference in the histopathological evaluation results between the ABS, MPH, and control groups. Our data indicate that both ABS and MPH represent safe, effective, and fast-acting hemostatic agents in the management of epistaxis. MPH was more effective than ABS in terms of hemostasis time and amount of bleeding.
Insights
Microporous polysaccharide hemospheres (MPH) and Ankaferd Blood Stopper (ABS) are safe and effective for managing epistaxis (nosebleeds). MPH demonstrated superior efficacy over ABS in reducing bleeding time and volume.
Area of Science:
- Otorhinolaryngology
- Biomaterials Science
- Experimental Medicine
Background:
- Epistaxis, or nosebleeds, poses a common clinical challenge.
- Effective hemostatic agents are crucial for managing nasal bleeding.
- Ankaferd Blood Stopper (ABS) and microporous polysaccharide hemospheres (MPH) are topical hemostatic agents with potential applications in epistaxis management.
Purpose of the Study:
- To evaluate and compare the histopathological impact, effectiveness, and safety of ABS and MPH in a rabbit epistaxis model.
- To assess the hemostatic efficacy of ABS and MPH in terms of hemostasis time and bleeding volume.
Main Methods:
- A standardized experimental epistaxis model was established in rabbits.
- Rabbits were randomized into three groups: control (saline), ABS-treated, and MPH-treated.
- Hemostasis time, bleeding amount, and histopathological changes were assessed post-treatment.
Main Results:
- Both ABS and MPH significantly reduced hemostasis time and bleeding amount compared to the control group (p = 0.004).
- MPH demonstrated superior effectiveness, significantly reducing hemostasis time (71.7 s) and bleeding amount (11.5 mg) compared to ABS (104.2 s, 20.5 mg) (p = 0.013 and p = 0.004, respectively).
- Histopathological evaluation revealed no significant differences among the groups, indicating comparable safety profiles.
Conclusions:
- Both Ankaferd Blood Stopper and microporous polysaccharide hemospheres are safe and effective hemostatic agents for epistaxis management.
- Microporous polysaccharide hemospheres exhibit greater efficacy than Ankaferd Blood Stopper in controlling nasal bleeding.
- Further clinical studies are warranted to confirm these findings in human patients.
