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The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...

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Related Experiment Video

Updated: Apr 8, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Extended KRAS and NRAS Mutation Profiling by Pyrosequencing®.

¹Pathologisches Institut, Ludwig-Maximilians-Universität, Thalkirchnerstrasse 36, 80337, Munich, Germany, Andreas.jung@lmu.de.

Methods in Molecular Biology (Clifton, N.J.)

|June 25, 2015

Summary

This summary is machine-generated.

Testing for RAS gene mutations (KRAS/NRAS) is crucial for metastatic colorectal cancer patients. Identifying these mutations ensures patients receive appropriate anti-EGFR targeted therapies, avoiding ineffective treatments.

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Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Targeted therapies and biomarkers are revolutionizing cancer treatment.
Anti-EGFR antibodies are used for metastatic colorectal cancer but efficacy depends on RAS gene status.

Purpose of the Study:

To outline a molecular-pathological testing protocol for RAS gene mutations.
To highlight the importance of RAS mutation status in guiding anti-EGFR therapy for colorectal cancer.

Main Methods:

Pyrosequencing® is presented as a robust method for detecting RAS gene mutations.
Focus on hotspot mutations in KRAS and NRAS genes (exons 2-4).

Main Results:

RAS gene mutations (KRAS/NRAS) confer resistance to anti-EGFR therapies.
Molecular testing identifies patients who will not benefit from these targeted drugs.

Conclusions:

RAS gene mutation testing is essential for personalized treatment strategies in metastatic colorectal cancer.
Pyrosequencing® provides an efficient method for this critical biomarker analysis.