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Updated: Apr 8, 2026
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Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
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Phage and Yeast Display
Jared Sheehan1, Wayne A Marasco1
1Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215.
Microbiology Spectrum
|June 25, 2015
Summary
Novel monoclonal antibodies (mAbs) offer effective alternatives to antibiotics for treating infections. Microbial display technologies like phage and yeast display enable rapid discovery of these therapeutic antibodies for infectious diseases.
Area of Science:
- Biotechnology
- Immunology
- Infectious Diseases
Background:
- Antimicrobial resistance is a growing clinical challenge, limiting the effectiveness of existing drugs.
- Novel therapeutic monoclonal antibodies (mAbs) present a promising alternative for treating and preventing infectious diseases.
- Human mAb therapies offer high specificity, minimizing impact on the microbiome and reducing resistance development in non-target species.
Purpose of the Study:
- To explore the potential of human monoclonal antibodies (mAbs) as alternatives to conventional antimicrobial drugs.
- To highlight microbial display technologies for the discovery of therapeutic mAbs against infectious pathogens.
- To discuss the application of phage and yeast display in developing mAbs for treatment and vaccines.
Main Methods:
- Utilizing in vitro selection of human antibody fragment libraries displayed on phage or yeast surfaces.
- Leveraging microbial surface display for large-scale generation and selection of antibody fragments.
- Focusing on phage display and yeast display technologies for antibody discovery.
Main Results:
- Microbial display technologies allow for the selection of human mAbs against infectious pathogens and toxins.
- These methods enable rapid isolation of antibody genes, overcoming limitations of traditional systems like murine hybridomas.
- The large repertoire of antibody fragments in single selection campaigns accelerates discovery.
Conclusions:
- Human mAbs generated through microbial display are viable therapeutic options for infectious diseases.
- Phage and yeast display are powerful tools for discovering novel mAbs for prophylaxis and treatment.
- These technologies are crucial for advancing vaccine development and combating antimicrobial resistance.
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