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Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
Published on: November 23, 2019
Selective sensing of pyrophosphate in physiological media using zinc(II)dipicolylamino-functionalised peptides
Vincent E Zwicker1, Benjamin M Long, Katrina A Jolliffe
1School of Chemistry, The University of Sydney, 2006, NSW, Australia. kate.jolliffe@sydney.edu.au.
Abstract:
A series of linear peptide based anion receptors, in which the distance between the bis[zinc(II)dipicolylamine] binding sites and the peptide backbone was varied systematically, was prepared and their anion binding ability was investigated using indicator displacement assays. Shortening the distance between the binding site and the peptide backbone was found to enhance both the receptor affinity and selectivity for pyrophosphate over other organic polyphosphate anions in Krebs buffer with the maximum selectivity and affinity observed with a spacer length of two methylene units. The suitability of these receptors for the determination of pyrophosphate concentrations in Krebs buffer and in artificial urine was examined.
