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Related Experiment Video

Updated: Apr 8, 2026

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Published on: April 24, 2018

A high-throughput assay for quantifying appetite and digestive dynamics.

Josua Jordi¹, Drago Guggiana-Nilo², Edward Soucy³

¹Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; and josuajordi@fas.harvard.edu.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology

|June 26, 2015

Summary

This summary is machine-generated.

Researchers developed a new method to track food intake and digestion in zebrafish larvae. This technique reveals how larvae regulate nutrient absorption based on demand and meal size, aiding disease research.

Keywords:

DiR' dye appetite hunger satiation satiety

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Area of Science:

Physiology
Neuroscience
Developmental Biology

Background:

Food intake and digestion are crucial physiological processes implicated in numerous diseases.
Existing methods for quantifying feeding and digestion lack scalability and dynamic measurement in unrestrained vertebrates.

Purpose of the Study:

To develop a novel method for high-throughput, dynamic quantification of feeding behavior and intestinal nutrient metabolism in zebrafish larvae.
To investigate nutrient intake regulation and digestive adjustments in response to demand and meal size in a vertebrate model.

Main Methods:

Integration of an infrared macroscope with fluorescently labeled food for precise tracking.
Utilizing zebrafish larvae as a model organism for studying feeding and digestion in naturally behaving subjects.
Application of rate-based modeling to analyze temporal dynamics of nutrient intake and metabolism.

Main Results:

Demonstrated that zebrafish larvae precisely match nutrient intake to their metabolic demands.
Showed that digestive rates adapt dynamically based on the size of the ingested meal.
Validated the model's translational relevance by observing mammalian-analogous responses to nicotine, l-lysine, ghrelin, and insulin.

Conclusions:

The developed method enables large-scale, high-resolution quantification of feeding and digestion in behaving vertebrates.
This approach facilitates the identification of chemical modulators of appetite and digestive processes.
The study advances translational research on food intake and digestive functions, with implications for understanding and treating related human diseases.