A simple flow injection analysis for studies of drug-protein binding interactions in serum

Studies of drug-protein binding constants (ka) and of the degree of binding (%B) in biological fluids have been carried out by means of flow injection analysis (FIA). The association constants found for clinically relevant concentrations agree with the literature data. The degree of binding found for discoumarol, phenytoin and sulfisoxazole is also in agreement with the literature whereas that of warfarin is slightly lower.